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An autoclave needs to be set at what temperature for how long to effectively sterilize?

  1. 200°F for 20 minutes

  2. 250°F for 30 minutes

  3. 300°F for 10 minutes

  4. 100°F for 40 minutes

The correct answer is: 250°F for 30 minutes

For effective sterilization in an autoclave, the standard requirement is to set the temperature to 250°F for at least 30 minutes. This specific temperature and time ensure that all forms of microbial life, including resistant spores, are killed. The high temperature of 250°F is essential because it allows steam to penetrate and sterilize equipment thoroughly, ensuring that all areas come in contact with the steam. The duration of 30 minutes at this temperature is widely accepted as adequate for achieving sterilization across most types of equipment used in tattooing, ensuring a safe and hygienic practice. This standard aligns with industry best practices and is crucial for maintaining health and safety in tattoo environments.